We’ve been using polysome profiling to measure and analyze mRNA translation in developing larvae. So far we’ve been looking at how nutrient/TOR signaling controls mRNA translation and examining how translational control of cell cycle genes may couple TOR signaling to endocycles.
In the future, we will use polysome profiling to ask the following:
- What mRNAs are translationally-regulated by nutrient deprivation?
- Do other environmental stresses affect mRNA translation in larvae? How? What mRNAs are regulated?